P.A.S. - 1714

The PAS kit is indicated for the visualization of tissue compounds characterized by adjacent glycol or aminohydroxyl groups.

In PAS staining, periodic acid selectively attacks the hydroxyl, 1,2-glycolic, hydroxyamino, and primary and secondary amino groups, causing the bond between two adjacent carbon atoms to break and the release of the aldehyde group, which is then detected with Schiff reagent.

Tissue compounds, which possess these free groups and in quantities sufficient to be detected, are polysaccharides, mucins, glycoproteins, glycopeptides, glycosaminoglycans, glycolipids, but also unsaturated lipids and phospholipids (such as sphingomyelin) which undergo oxidation of conjugated double bonds with the formation of two hydroxyl groups which are further oxidized to aldehydes.